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This article is mentioned in:. We found that: i the circRNA expression profile revealed 1, significant differences in circRNA expression, with circRNA expression downregulated in samples and upregulated in samples via interactions with miRNAs. Among them, 2, differentially expressed genes were upregulated and 3, genes were downregulated.
Gene Ontology GO analysis of the differentially expressed genes revealed these genes involved in biological process classification, cellular component classification and molecular function classification. Pathway analysis of the differentially expressed genes identified 83 significantly enriched genes, including 28 upregulated genes and 55 downregulated genes.
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These circRNAs regulated the expression of target genes through interactions with miRNAs and might become new molecular biomarkers for GC in the future. Introduction Gastric cancer GC is a malignant tumor that occurs in the gastric mucosa of the digestive tract 1. Materials and methods Human subjects: Patients and healthy controls The 8 GC tissues and adjacent tissue samples used in this study were collected from the Shenzhen People's Hospital.
Tissue sample collection GC and adjacent tissues 1 cm 3 obtained after stomach cancer surgeries were immediately washed with 0. Screening and identification of gastric cancer-associated circRNAs Sample labeling and array hybridization were performed according to the manufacturers protocol Arraystar Inc. Screening and identification of gastric cancer-associated mRNAs Human whole genome oligonucleotide microarrays representing all known genes and transcripts in the human gene were applied in the extensive view.
Differentially expressed gene analysis and functional analysis We used the Agilent Feature Extraction v Data acquisition and analysis Scanned images were imported into the Agilent Feature Extraction software for raw data extraction. Scatter plot of differentially expressed circRNAs. Verification of the circRNA microarray results.
Scatter plot of the differentially expressed genes. The verification results of differential gene expression. GO biological process classifications of upregulated genes. GO molecular function classifications of downregulated genes. The main pathways. Related Articles. Tweets by OncologyReports. Follow OncologyReports.
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